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This assay included sequential injections of 2 µM Oligomycin (to inhibit ATP synthase (complex V)) and 2 µM FCCP (Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone) (to uncouple oxidative phosphorylation by dissipating the proton gradient, allowing for the measurement of maximal respiration) to measure mitochondrial function and the oxygen consumption rate (OCR)