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The process of tuning and calibration is critical to achieve optimum LC–MS results, as this process sets: Voltages on the source elements AMU gain and offset for the correct peak width The electron multiplier voltage The mass axis for proper mass assignment Tuning and calibration is important as it: Calibrates the m/z scale of the spectrometer versus standards of known m/z ratio Provides the means (if performed on a periodic basis) to realize that spectrometer contamination or degraded electronic components have changed the selected mass positions Ensures repeatable analysis from instrument to instrument or lab to lab Checks that spectrometer gives expected relative ratios of ion fragment intensities for a target compound Acts as a diagnostic tool to indicate the service/cleaning requirements of the spectrometer Provides results that act as a chronicle of system performance Calibration Mass calibration is carried out by infusing a standard with a well-known mass spectrum into the LC-MS instrument and adjusting the response of the MS components accordingly