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Additionally, the nuclear transcription factor (Foxo1) is phosphorylated upon activation of phosphoinositide- 3-kinase–protein kinase B/Akt (PI3 K-PKB/Akt), which in turn deactivates molecules to restrict the activity of proapoptotic pathways [66]
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The stained cells were analyzed using a FACSCalibur system (Becton Dickinson)
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