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Phase 2 enzymes include several superfamilies of conjugating enzymes, such as the glutathione-S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), sulfotransferases (SULTs), N -acetyltransferases (NATs), and methyltransferases (MTs)
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Reactions (20 ΞΌL) were run in technical triplicates under the following conditions: 95Β°C for 10 min, followed by 40 cycles of 95Β°C for 15 s and 60Β°C for 1 min, and a melt curve (65β95Β°C)
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