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(17)制备粗品 (17) Preparation of crude product 把吹干的Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin倒入玻璃的切割瓶中，加入预冷的切肽试剂(TFA9.5ml，H 2 O0.5ml)，常温反应1.5小时，过滤除去树脂，加冰乙醚500ml沉淀，3500转3分钟离心收集沉淀物，用乙醚洗涤3次，用纯水溶解后，低温冷冻干燥24小时，获 得BPC157肽粗品1.23g，经HPLC测定纯度为78％。 Pour the dried Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin into the glass Add pre-cooled peptide cutting reagent (TFA9.5ml, H 2 O0.5ml) to the cutting bottle, react at room temperature for 1.5 hours, filter to remove the resin, add 500ml of glacial ether to precipitate, centrifuge at 3500 for 3 minutes to collect the precipitate, and diethyl ether After washing 3 times, dissolving with pure water, and lyophilizing at low temperature for 24 hours, 1.23 g of crude product of BPC157 peptide was obtained, and the purity was 78% as determined by HPLC