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The MSMS spectra (Figure S7b) clearly showed that the C-terminal amino acid, Cys, was the one that incorporated a deuteron since all the fragment ions from the peptide originated from the D 2 O-labeled rituximab were 1 Da heavier than those originated from the peptide originated from the H 2 O-labeled rituximab, unequivocally showing that the Cys is the C-terminal amino acid of the light chain

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