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Afterward, 0.5 µg of sequencing grade trypsin (Promega), diluted in 50 mM ammonium bicarbonate, was added into the sample and digested at room temperature for 18 h

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Excretion Pathways Limited pharmacokinetic data indicate peptide fragment elimination through standard renal and hepatic routes: Renal excretion of peptide metabolites as primary elimination pathway Hepatic metabolism contributing to peptide degradation Complete elimination within approximately 60 minutes based on half-life calculations No evidence of tissue accumulation or depot formation The dissociation between rapid plasma clearance and prolonged growth hormone elevation demonstrates that sermorelin acts as a secretagogue trigger rather than requiring sustained receptor occupancy