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Figure 4 Figure 5 3.5 Cystine increases intracellular ferrous ion concentrations and elevates ROS levels following gentamicin treatment Despite their specific drug–target interactions, bactericidal antibiotics are known to stimulate the production of highly deleterious hydroxyl radicals in bacteria, a process that is facilitated through the Fenton reaction involving intracellular ferrous iron (Kohanski et al., 2007)