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Enhancing these transporters can increase the availability of key precursors for neurotransmitter production, contributing to neuronal health and further reinforcing BBB integrity [158,159,505]
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Two of the three pellets were resuspended in 40 ฮผl of lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1% Triton, 1x PIC), while the third pellet was resuspended in 1X glyco-buffer 2 (50 mM sodium phosphate pH 7.5, 1% NP-40, 1X PIC)
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