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Quantitative accuracy is enhanced through incorporation of stable isotope-labeled synthetic peptides as internal standards (Liquid Chromatography-Stable Isotope Dilution-MRM MS or LC-SID-MRM MS), a methodology successfully applied for absolute quantification of CFTR protein using signature peptides as full-length protein surrogates (McShane et al., 2014)
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On an immunomodulatory level, anti-inflammatory factors secreted by the amniotic membrane (e.g., SLPI/elafin) synergize with MenSC-derived paracrine cytokines such as IDO, PGE 2 , and TGF-β, promoting the polarization of macrophages from the pro-inflammatory M1 phenotype to the regenerative M2 phenotype
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