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The resulting fermentation-enriched metabolites act on multiple nodes of the gut–immune axis, synergistically suppressing pro-inflammatory factors (e.g., TNF-α) and enhancing antioxidant enzyme activity (e.g., SOD), thereby more effectively mitigating colitis
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HT29 cells were cultured in DMEM supplemented with 10% Foetal Bovine Serum (FBS) and 1% penicillin–streptomycin in a humidified cell incubator with 5% CO 2 at 37 °C
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