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Isolates that no longer could grow in the presence of gentamicin were screened for deletion of the wild-type allele using the primer pair PA2140_Tn7_F/R by colony PCR (Figure 1B) and assessed for correctness by Sanger sequencing

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In addition, as cyclin D can be repressed by a paralog of SIM from rice when expressed in yeast, this suggests that CYCD3;1 may be a second target of SIM [111] (Fig