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When a paracellular route is intentionally targeted, a brief and reversible opening of tight junctions is required in which calcium chelators can lower extracellular calcium and loosen cadherin complexes, and medium-chain fatty acids or bile salts can disturb membrane order and temporarily reorganize claudin and occludin
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For RT-qPCR, RNA was reverse-transcribed using PrimeScript Reverse Transcription Supermix (Bio-Rad)
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