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Using GC-MS, the researchers also showed that the most abundant amino acid in bull seminal fluid was glutamic acid

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The millimolar GSH intracellular concentrations, the low plasma micromolar concentrations and the low GSH redox potential ( E ′ 0 = −240 mV) make GSH an ideal and perfect cellular redox buffer (Figure 2) ( FIGURE 1 FIGURE 2 Glutathione is synthesized in the cytosol of all cells from their precursor amino acids: glutamic acid, cysteine and glycine by consecutive action of two enzymes: γ-glutamyl-cysteine (γ-GluCys) synthetase (also known as glutamate cysteine ligase, GCL) that in a first step uses glutamate and cysteine as a substrate to form the dipeptide γ-glutamyl-cysteine