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In order to ensure that the cells recovered at 24 h originated from cells that had been permeabilized and exposed to Xenopus egg factors, the presence of Lamin B3 was checked for after the resealing phase
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282,283 While Met790 oxidation is reversible, Cys797 oxidation on EGFR T790M further upregulates EGFR-NOX2, which increases ROS production, resulting in aberrant EGFR expression
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