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Examples of the additional agents that are not GLP-1 receptor agonists which may be used in conjunction with a biguanide include, without limitation: myostatin inhibitors (e.g., selective or non-selective inhibitors of myostatin), glucagon receptor agonists, GIP receptor agonists, peroxisome proliferator-activated receptor (PPAR) alpha agonists (such as fibrates), alpha-glucosidase inhibitors, dipeptidyl peptidase-4 (DPP-4) inhibitors, sodium-glucose transporter (SGLT) 2 inhibitors, sulfonylurea, meglitinide, thiazolidinedione, FGF21 receptor agonists, FGF19 receptor agonists, mucin-complexing polymers (such as GLY-200 being developed by Glyscend Tx), did inhibitors and/or mitochondrial decoupling agents (e.g., controlled metabolic accelerators such as HU6 being myostatin inhibitors the additional agent used in conjunction with a biguanide is a myostatin inhibitor

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NO, most extensively, has been shown to serve as a mediator in various biological processes